Lipid peroxidation and total radical-trapping potential of the lungs of rats submitted to chronic and sub-chronic stress

Brazilian Journal of Medical and Biological Research

R.l. Torres¹  I.l.s. Torres²  G.d. Gamaro²  F.u. Fontella¹  P.p. Silveira²  J.s.r. Moreira²  M. Lacerda²  J.r. Amoretti²  D. Rech²  C. Dalmaz²  A.a. Belló¹ 

[1] ,Universidade Federal do Rio Grande do Sul Instituto de Ciências Básicas da Saúde Departamento de Bioquímica

关键词: Stress;    TBARS;    TRAP;    Free radicals;    Lungs;    Oxidative stress;   

DOI  :  10.1590/S0100-879X2004000200004

来源: SciELO

【 摘 要 】

Exposure to stress induces a cluster of physiological and behavioral changes in an effort to maintain the homeostasis of the organism. Long-term exposure to stress, however, has detrimental effects on several cell functions such as the impairment of antioxidant defenses leading to oxidative damage. Oxidative stress is a central feature of many diseases. The lungs are particularly susceptible to lesions by free radicals and pulmonary antioxidant defenses are extensively distributed and include both enzymatic and non-enzymatic systems. The aim of the present study was to determine lipid peroxidation and total radical-trapping potential (TRAP) changes in lungs of rats submitted to different models of chronic stress. Adult male Wistar rats weighing 180-230 g were submitted to different stressors (variable stress, N = 7) or repeated restraint stress for 15 (N = 10) or 40 days (N = 6) and compared to control groups (N = 10 each). Lipid peroxidation levels were assessed by thiobarbituric acid reactive substances (TBARS), and TRAP was measured by the decrease in luminescence using the 2-2'-azo-bis(2-amidinopropane)-luminol system. Chronic variable stress induced a 51% increase in oxidative stress in lungs (control group: 0.037 ± 0.002; variable stress: 0.056 ± 0.007, P < 0.01). No difference in TBARS was observed after chronic restraint stress, but a significant 57% increase in TRAP was presented by the group repeatedly restrained for 15 days (control group: 2.48 ± 0.42; stressed: 3.65 ± 0.16, P < 0.05). We conclude that different stressors induce different effects on the oxidative status of the organism.

【 授权许可】

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