Effect of one-week ethanol treatment on monoamine levels and dopaminergic receptors in rat striatum

Brazilian Journal of Medical and Biological Research

S.m.m. Vasconcelos¹  D.s. Macêdo¹  L.o. Lima¹  F.c.f. Sousa¹  M.m.f. Fonteles¹  G.s.b. Viana¹ 

[1] ,Universidade Federal do Ceará Faculdade de Medicina Departamento de Fisiologia e FarmacologiaFortaleza CE ,Brasil

关键词: Rat striatum;    Dopamine;    Serotonin;    Alcohol ingestion;    Noradrenaline;    D1 and D2 receptor density;    Kd;   

DOI  :  10.1590/S0100-879X2003000400013

来源: SciELO

【 摘 要 】

We studied the effects of ethanol on the levels of norepinephrine, dopamine, serotonin (5-HT) and their metabolites as well as on D1- and D2-like receptors in the rat striatum. Ethanol (2 or 4 g/kg, po) was administered daily by gavage to male Wistar rats and on the 7th day, 30 min or 48 h after drug administration, the striatum was dissected for biochemical assays. Monoamine and metabolite concentrations were measured by HPLC and D1- and D2-like receptor densities were determined by binding assays. Scatchard analyses showed decreases of 30 and 43%, respectively, in D1- and D2-like receptor densities and no change in dissociation constants (Kd) 48 h after the withdrawal of the dose of 4 g/kg. Ethanol, 2 g/kg, was effective only on the density of D2-like receptors but not on Kd of either receptor. Thirty minutes after the last ethanol injection (4 g/kg), decreases of D2 receptor density (45%) as well as of Kd values (34%) were detected. However, there was no significant effect on D1-like receptor density and a 46% decrease was observed in Kd. An increase in dopamine and 3,4-dihydroxyphenylacetic acid (DOPAC), a decrease in norepinephrine, and no alteration in 5-HT levels were demonstrated after 48-h withdrawal of 4 g/kg ethanol. Similar effects were observed in dopamine and DOPAC levels 30 min after drug administration. No alteration in norepinephrine concentration and a decrease in 5-HT levels were seen 30 min after ethanol (4 g/kg) administration. Our findings indicate the involvement of the monoaminergic system in the responses to ethanol.

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