Detection of virulence factors and antimicrobial resistance patterns in shiga toxin-producing Escherichia coli isolates from sheep

Pesquisa Veterinária Brasileira

Marcos R.a. Ferreira¹  Talícia Dos S. Silva¹  Ariel E. Stella¹  Fabricio R. Conceição¹  Edésio F. Dos Reis¹  Cecília N. Moreira¹ 

关键词: Escherichia coli;    STEC;    multiresistant isolates;    molecular characterization;    phylogenetic.;    Escherichia coli;    STEC;    isolados multirresistentes;    caracterização molecular;    filogenético.;   

DOI  :  10.1590/S0100-736X2015000900002

来源: SciELO

【 摘 要 】

Abstract: In order to detect virulence factors in Shiga toxin-producing Escherichia coli (STEC) isolates and investigate the antimicrobial resistance profile, rectal swabs were collected from healthy sheep of the races Santa Inês and Dorper. Of the 115 E. coli isolates obtained, 78.3% (90/115) were characterized as STEC, of which 52.2% (47/90) carried stx1 gene, 33.3% (30/90) stx2 and 14.5% (13/90) both genes. In search of virulence factors, 47.7% and 32.2% of the isolates carried the genes saa and cnf1. According to the analysis of the antimicrobial resistance profile, 83.3% (75/90) were resistant to at least one of the antibiotics tested. In phylogenetic classification grouped 24.4% (22/90) in group D (pathogenic), 32.2% (29/90) in group B1 (commensal) and 43.3% (39/90) in group A (commensal). The presence of several virulence factors as well as the high number of multiresistant isolates found in this study support the statement that sheep are potential carriers of pathogens threatening public health.

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