Identification of canine papillomavirus type 1 (CPV1) DNA in dogs with cutaneous papillomatosis

Pesquisa Veterinária Brasileira

Brígida K. De Alcântara¹  Amauri A. Alfieri¹  Wagner B. Rodrigues¹  Rodrigo A.a. Otonel¹  Michele Lunardi¹  Selwyn A. Headley²  Alice F. Alfieri¹ 

[1] ,Universidade Estadual de Londrina Departamento de Medicina Veterinária Preventiva Laboratório de Virologia AnimalLondrina PR ,Brazil

关键词: Papillomatosis;    canine;    CPV1;    papilloma;    warts;    L1 gene;    Papilomatose;    canina;    CPV1;    papiloma;    verrugas;    gene L1;   

DOI  :  10.1590/S0100-736X2014001200013

来源: SciELO

【 摘 要 】

Canine oral papillomavirus (COPV), also known as Canine Papillomavirus type 1 (CPV1), induces papillomas at the mucous membranes of the oral cavity and at the haired skin of dogs. The classification of Papillomavirus (PV) types is based on the L1 capsid protein and nucleotide sequence; so far, 14 CPV types have been described in several countries, but the molecular characterization of CPV in Brazil is lacking. This study investigated the presence of the PV in seven papillomas from four mixed breed dogs from Londrina/PR, Southern Brazil, by partial sequencing of the L1 gene. Seven exophytic cutaneous lesions were surgically removed and processed for histopathological and molecular characterization. Histopathology confirmed the lesions as viral papillomas due to typical histological features. Polymerase Chain Reaction (PCR) assay using the FAP59 and FAP64 primers targeted the L1 gene followed by sequence analysis of the amplicons identified CPV1 in all evaluated papilloma samples. This study represents the first description of CPV1 DNA associated with canine papillomatosis in Brazil.

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