Development of IgY antibodies in chickens and IgG in rabbits immunized against proteins of Pythium insidiosum isolated from horses in the state of Rio de Janeiro

Pesquisa Veterinária Brasileira

Maria Fabíola Nunes Rangel¹  Luciana Da Silva Lemos¹  Cláudia Maria Costa De Almeida¹  Lério Gama Sales¹  Olney Vieira-da Motta¹ 

关键词: Pythiosis;    Pythium insidiosum;    diseases of horses;    IgY and IgG antibodies;    Pitiose;    Pythium insidiosum;    doenças de equinos;    anticorpos IgY e IgG;   

DOI  :  10.1590/S0100-736X2010000100014

来源: SciELO

【 摘 要 】

Pythiosis is caused by Pythium insidiosum and the occurrence of disease in horses was described in the North and Northwest State of Rio de Janeiro, Brazil. The disease was described in cattle, sheep, humans, and horses in different states and regions across the country. This paper describes the development of IgY and IgG polyclonal antibodies, in chicken and rabbits, respectively against proteins extracted from kunkers and hyphae of P. insidiosum from affected horses. The proteins were recognized by chicken, rabbit and horse antibodies by immunodiffusion and Western blot against majority bands of 27 and 43 KDa, and titrated by ELISA. The antibodies IgY developed by the first time against Brazilian strains of P. insidiosum may represent a valuable tool in the detection of antigens of the pathogen and contribute to further studies aimed at immunotherapy and knowledge about this disease in endemic areas in Rio de Janeiro and in Brazil.

【 授权许可】

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