Hematological and immunological responses of Nile tilapia after polyvalent vaccine administration by different routes

Pesquisa Veterinária Brasileira

Bruno C. Silva²  Mauricio L. Martins¹  Adolfo Jatobá²  Celso C. Buglione Neto²  Felipe N. Vieira²  Gabriella V. Pereira²  Gabriela T. Jerônimo¹  Walter Q. Seiffert²  José Luiz P. Mouriño² 

[1] ,Universidade Federal de Santa Catarina Centro de Ciências Agrárias Departamento de AqüiculturaFlorianópolis SC ,Brasil

关键词: Oreochromis niloticus;    vaccine;    hematology;    immunology;    Oreochromis niloticus;    vacina;    hematologia;    imunologia;   

DOI  :  10.1590/S0100-736X2009001100002

来源: SciELO

【 摘 要 】

The efficacy of a polyvalent bacterin vaccine against Aeromonas hydrophila, Pseudomonas aeroginosa and Enterococcus durans administered by different routes in Nile tilapia was assessed by analyzing hematological and immunological parameters 7 and 21 days after vaccination. Treatments consisted of: non-vaccinated tilapia; tilapia vaccinated by intraperitoneal injection with 2x10(8) formalin-inactivated bacteria·mL-1; tilapia vaccinated orally with 2x10(7) formalin-inactivated bacteria·g-1, feed for 5 days; tilapia vaccinated by immersion bath in 2x10(7) formalin-inactivated bacteria·mL-1, for 20 minutes. Vaccinated fish groups presented higher hematocrit, number of erythrocytes and leukocytes than the non-vaccinated group. Serum agglutination titer of intraperitoneally vaccinated fish was higher on both evaluation periods for the three bacteria strains. Only on day 21 post-vaccination fish from the oral and immersion vaccination groups presented higher serum agglutination titer than the non-vaccinated fish for A. hidrophyla and E. durans. Serum antimicrobial activity in vaccinated fish was higher for P. aeroginosa and E. coli than in non-vaccinated fish on both evaluation periods. The different vaccine administration routes stimulated hematological and immunological responses in Nile tilapia 21 days post-vaccination, but intraperitoneal vaccination presented higher total number of leukocytes, lymphocytes and serum agglutination titer.

【 授权许可】

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