Hematological and immunological responses of Nile tilapia after polyvalent vaccine administration by different routes

Pesquisa Veterinária Brasileira

Martins, Mauricio L.²  Buglione Neto, Celso C.¹  Vieira, Felipe N.¹  Pereira, Gabriella V.¹  Jatobá, Adolfo¹  Mouriño, José Luiz P.¹  Seiffert, Walter Q.¹  Jerônimo, Gabriela T.²  Silva, Bruno C.¹ 

[1] Universidade Federal de Santa Catarina, Florianópolis, Brasil;UFSC, Florianópolis

关键词: Oreochromis niloticus;    vaccine;    hematology;    immunology.;   

DOI  :  10.1590/S0100-736X2009001100002

来源: Colegio Brasileiro de Patologia Animal-CBPA

【 摘 要 】

The efficacy of a polyvalent bacterin vaccine against Aeromonas hydrophila, Pseudomonas aeroginosa and Enterococcus durans administered by different routes in Nile tilapia was assessed by analyzing hematological and immunological parameters 7 and 21 days after vaccination. Treatments consisted of: non-vaccinated tilapia; tilapia vaccinated by intraperitoneal injection with 2x108 formalin-inactivated bacteria·mL-1; tilapia vaccinated orally with 2x107 formalin-inactivated bacteria·g-1, feed for 5 days; tilapia vaccinated by immersion bath in 2x107 formalin-inactivated bacteria·mL-1, for 20 minutes. Vaccinated fish groups presented higher hematocrit, number of erythrocytes and leukocytes than the non-vaccinated group. Serum agglutination titer of intraperitoneally vaccinated fish was higher on both evaluation periods for the three bacteria strains. Only on day 21 post-vaccination fish from the oral and immersion vaccination groups presented higher serum agglutination titer than the non-vaccinated fish for A. hidrophyla and E. durans. Serum antimicrobial activity in vaccinated fish was higher for P. aeroginosa and E. coli than in non-vaccinated fish on both evaluation periods. The different vaccine administration routes stimulated hematological and immunological responses in Nile tilapia 21 days post-vaccination, but intraperitoneal vaccination presented higher total number of leukocytes, lymphocytes and serum agglutination titer. Index

【 授权许可】

CC BY-NC