Serological diagnosis of visceral leishmaniasis by an enzyme immunoassay using protein A in naturally infected dogs

Pesquisa Veterinária Brasileira

Valéria Marçal Felix De Lima²  Luciane Biazzono¹  Ana Cláudia Silva¹  Ana Paula Ferreira Lopes Correa¹  Maria Cecília Rui Luvizotto¹ 

[1] ,Universidade Estadual Paulista Faculdade de Medicina Veterinária de Araçatuba Departamento de Clínica, Cirurgia e Reprodução Animal,Araçatuba SP ,Brazil

关键词: Leishmania chagasi;    visceral leishmaniasis;    protein A;    dogs;    Leishmania chagasi;    ELISA;    leishmaniose visceral;    cães;   

DOI  :  10.1590/S0100-736X2005000400005

来源: SciELO

【 摘 要 】

A rapid indirect enzyme-linked immunosorbent assay (ELISA) was developed for measuring antibodies against Leishmania chagasi using total antigen from lysed promastigotes. Fifty symptomatic mixed breed dogs from a region of high incidence of visceral leishmaniasis in Brazil were examined. The results showed that in the positive animals, diagnosed by cytological examination, the ELISA using protein A assay system (mean optical density ± SD / 2.078 ± 0.631) detected more antibodies than the anti-IgG assay (mean optical density ± SD / 1.008 ± 0.437), while in the negative animals, the results by both systems were similar. These results suggest that the ELISA assay using protein A peroxidase conjugated could be useful to detect early infected animals in endemic areas, and thus help to control the spread of the infection.

【 授权许可】

CC BY-NC   
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