Avaliação de seis testes sorológicos no diagnóstico da brucelose bubalina

Pesquisa Veterinária Brasileira

László Molnár¹  Éva Molnár¹  Emilia S.c. Lima¹  Hilma L.t. Dias¹ 

[1] ,Universidade Federal do Pará Centro Agropecuário Laboratório de Investigação e Diagnóstico de Enfermidades Animal

关键词: Brucellosis;    water buffalo;    competitive ELISA;    indirect ELISA;    agglutination;    Bucelose;    bubalino;    ELISA competitivo;    ELISA indireto;    aglutinação;   

DOI  :  10.1590/S0100-736X2002000200002

来源: SciELO

【 摘 要 】

Four hundred and forty buffalo sera, selected from about 1,200 blood samples of another study, were examined. The samples were tested by six serological methods: two of agglutination, two of indirect ELISA and two of competitive ELISA. To determine the relative sensitivity and specificity of different tests, animals with a positive result to competitive ELISA of the FAO/IAEA were considered as infected. The relative sensitivity of competitive ELISA, indirect ELISA with conjugate anti-bovine light chain monoclonal antibody labelled with HRPO, indirect ELISA with anti-bovine IgG conjugate, rose Bengal test and rapid slide agglutination test was 100%, 98.57%, 97.14%, 91.42% and 79.28%, and the relative specificity 99.33%, 97.33%, 95.66%, 94.00% and 86.33%, respectively. The value of the different serological tests for the diagnosis of brucellosis is discussed.

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