Stability-indicating RP-HPLC method for simultaneous determination of gatifloxacin and flurbiprofen in binary combination

Química Nova

Islam Ullah Khan²  Syed Naeem Razzaq²  Irfana Mariam¹  Muhammad Ashfaq¹  Syed Saleem Razzaq¹ 

[1] ,Government College University Department of Chemistry Lahore,Pakistan

关键词: gatifloxacin;    flurbiprofen;    degradation products;   

DOI  :  10.5935/0100-4042.20140058

来源: SciELO

【 摘 要 】

A stability-indicating RP-HPLC method is presented for determination of gatifloxacin and flurbiprofen in binary combination. Gatifloxacin, flurbiprofen and their degradation products were detected at 254 nm using a BDS Hypersil C8 (250 X 4.6 mm, 5 µm) column and mixture of 20 mM phosphate buffer (pH 3.0) and methanol 30:70 v/v as mobile phase. Response was linear over the range of 15-105 mg mL-1 for gatifloxacin (r² > 0.998) and of 1.5-10.5 mg mL-1 for flurbiprofen (r² > 0.999). The developed method efficiently separated the analytical peaks from degradation products (peak purity index > 0.9999). The method developed can be applied successfully for determination of gatifloxacin and flurbiprofen in human serum, urine, pharmaceutical formulations, and their stability studies.

【 授权许可】

CC BY-NC   
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