Stability-indicating RP-HPLC method for simultaneous determination of gatifloxacin and flurbiprofen in binary combination

Quimica nova

Khan, Islam Ullah¹  Razzaq, Syed Naeem¹  Razzaq, Syed Saleem²  Mariam, Irfana³  Ashfaq, Muhammad⁴ 

[1] Government College University, Lahore, Pakistan;Medipharm Pharmaceuticals Kot Lakhpat, Lahore, Pakistan;Queen Marry College, Lahore, Pakistan;University of Gujrat, Gujrat, Pakistan

关键词: gatifloxacin;    flurbiprofen;    degradation products.;   

DOI  :  10.5935/0100-4042.20140058

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

A stability-indicating RP-HPLC method is presented for determination of gatifloxacin and flurbiprofen in binary combination. Gatifloxacin, flurbiprofen and their degradation products were detected at 254 nm using a BDS Hypersil C8 (250 X 4.6 mm, 5 µm) column and mixture of 20 mM phosphate buffer (pH 3.0) and methanol 30:70 v/v as mobile phase. Response was linear over the range of 15-105 mg mL-1 for gatifloxacin (r2 > 0.998) and of 1.5-10.5 mg mL-1 for flurbiprofen (r2 > 0.999). The developed method efficiently separated the analytical peaks from degradation products (peak purity index > 0.9999). The method developed can be applied successfully for determination of gatifloxacin and flurbiprofen in human serum, urine, pharmaceutical formulations, and their stability studies.

【 授权许可】

CC BY