Otimização da esterificação de ácido hexanóico com n-butanol empregando lipase (Termomyces lanuginosus) imobilizada em gelatina

Química Nova

Everton Skoronski²  Jair Juarez João²  Maria Alice Prado Cechinel¹  Mylena Fernandes¹ 

[1] ,Universidade do Estado de Santa Catarina Departamento de Engenharia Ambiental Lages SC ,Brasil

关键词: immobilization;    lipase;    esther synthesis;   

DOI  :  10.1590/S0100-40422013000300003

来源: SciELO

【 摘 要 】

The application of Lipozyme (Termomyces lanuginosus) immobilized in gelatin gel in aliphatic ester synthesis was investigated taking the esterification of hexanoic acid with n-butanol as a model reaction. Conditions were optimized by factorial design and the highest conversion was obtained under the following conditions: molar ratio alcohol: acid of 2:1, reaction time of 48 h and biocatalyst weight of 7.0 g. Under these conditions the esterification yield was around 98 %. The operational stability of the immobilized lipase was assessed and results showed that after 12 batch runs, the enzyme showed no significant loss of activity.

【 授权许可】

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