Desempenho da matriz híbrida SiO2-quitosana na imobilização da lipase microbiana de Candida rugosa

Química Nova

Aline S Simões²  Rodrigo Y Mori²  Raquel Faria²  Heizir F. De Castro²  Adriano A Mendes¹ 

[1] ,Universidade de São Paulo Escola de Engenharia de Lorena Lorena SP ,Brasil

关键词: immobilization;    lipase;    SiO2-chitosan;   

DOI  :  10.1590/S0100-40422011000100007

来源: SciELO

【 摘 要 】

Lipase from Candida rugosa was immobilized by covalent attachment on hybrid SiO2-chitosan obtained by sol-gel technique. A comparative study between free and immobilized lipase was provided in terms of pH, temperature, kinetic parameters and thermal stability on the olive oil hydrolysis. The pH and temperature for maximum activity shifted from 7.0 and 45 ºC for the free lipase to 7.5 and wide range of temperature (40-50 ºC) after immobilization. Kinetics parameters were found to obey Michaelis-Menten equation and K M values indicated that immobilization process reduced the affinity of enzyme-substrate; however Kd values revealed an increase of thermal stability of lipase.

【 授权许可】

CC BY-NC   
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