Biochemical properties of Bacillus sp. ITP-001 lipase immobilized with a sol gel process

Química Nova

Nayara Bezerra Carvalho¹  José Murillo P. Barbosa¹  Maria Vanessa S. Oliveira¹  Alini T. Fricks¹  Álvaro S. Lima¹  Cleide Mara F. Soares¹ 

[1] ,Instituto de Tecnologia e PesquisaAracaju SE ,Brasil

关键词: biochemical;    lipase from Bacillus immobilized;    sol gel process;   

DOI  :  10.1590/S0100-40422013000100010

来源: SciELO

【 摘 要 】

This work presents biochemical characterization of a lipase from a new strain of Bacillus sp. ITP-001, immobilized using a sol gel process (IB). The results from the biochemical characterization of IB showed increased activity for hydrolysis, with 526.63 U g-1 at pH 5.0 and 80 ºC, and thermal stability at 37 ºC. Enzymatic activity was stimulated by ions such as EDTA, Fe+3, Mn+2, Zn+2, and Ca+2, and in various organic solvents. Kinetic parameters obtained for the IB were Km = 14.62 mM, and Vmax = 0.102 mM min-1 g-1. The results of biochemical characterization revealed the improved catalytic properties of IB.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License