Biochemical properties of Bacillus sp. ITP-001 lipase immobilized with a sol gel process

Química Nova

Soares, Cleide Mara F.¹  Barbosa, José Murillo P.¹  Lima, Álvaro S.¹  Instituto de Tecnologia e Pesquisa, Aracaju, Brasil¹  Carvalho, Nayara Bezerra¹  Fricks, Alini T.¹  Oliveira, Maria Vanessa S.¹ 

关键词: biochemical;    lipase from Bacillus immobilized;    sol gel process.;   

DOI  :  10.1590/S0100-40422013000100010

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

This work presents biochemical characterization of a lipase from a new strain of Bacillus sp. ITP-001, immobilized using a sol gel process (IB). The results from the biochemical characterization of IB showed increased activity for hydrolysis, with 526.63 U g-1 at pH 5.0 and 80 ºC, and thermal stability at 37 ºC. Enzymatic activity was stimulated by ions such as EDTA, Fe+3, Mn+2, Zn+2, and Ca+2, and in various organic solvents. Kinetic parameters obtained for the IB were Km = 14.62 mM, and Vmax = 0.102 mM min-1 g-1. The results of biochemical characterization revealed the improved catalytic properties of IB.

【 授权许可】

Unknown