Química Nova | |
Sensitive method for determination of piplartine, an alkaloid amide from Piper species, in rat plasma samples by liquid chromatography-tandem mass spectrometry | |
Daniel P. Bezerra1  Cláudia Pessoa1  Manoel O. Moraes2  Letícia V. Costa-lotufo1  Dayana Rubio Gouvea2  Valquíria A. Polisel Jabor2  Norberto Peporine Lopes2  Keyller Bastos Borges2  Mary Anne S. Lima2  Edilberto R. Silveira2  | |
[1] ,Universidade Federal do Ceará Faculdade de Medicina Departamento de Fisiologia e FarmacologiaFortaleza CE ,Brasil | |
关键词: piplartine; LC-MS; rat plasma analysis; | |
DOI : 10.1590/S0100-40422012000300004 | |
来源: SciELO | |
【 摘 要 】
Piplartine (PPTN) is an alkaloid amide found in Piper species that presents different activities. PPTN determination in rat plasma is necessary to better understand its biological effects. The aim of this study was to develop a sensitive LC-MS/MS method for the determination of PPTN in rat plasma. The performance criteria for linearity, sensitivity, precision, accuracy, recovery, and stability have been assessed and were within the recommended guidelines. The validated method proved to be suitable in a pilot study of PPTN kinetic disposition in rat plasma after a single intraperitoneal dose, and represents an appropriate tool to further pharmacokinetic studies.
【 授权许可】
CC BY-NC
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
【 预 览 】
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RO202005130063839ZK.pdf | 401KB | download |