期刊论文详细信息
Química Nova
A sensitive and robust lc-ms/ms method with monolithic column and electrospray ionization for the quantitation of efavirenz in human plasma: application to a bioequivalence study
Danilo Cesar Galindo Bedor1  Jose Homero De Souza Filho1  Virna Ligiane Souza Ramos1  Talita Mota Gonçalves1  Carlos Eduardo Miranda De Sousa1  Davi Pereira De Santana1 
[1] ,Universidade Federal de Pernambuco Departamento de Ciências Farmacêuticas Recife PE ,Brasil
关键词: efavirenz;    bioequivalence;    LC-MS/MS;   
DOI  :  10.1590/S0100-40422011000600007
来源: SciELO
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【 摘 要 】

An LC-MS/MS method has been developed for the determination of efavirenz (EFZ) in human plasma using hydrochlorothiazide as internal standard (I.S.). An ESI negative mode with multiple reaction-monitoring was used monitoring the transitions m/z 313.88→69.24 (EFZ) and 296.02→204.76 (I.S.). Samples were extracted using liquid-liquid extraction. The total run time was 2.0 min. The separation was achieved with HPLC-RP using a monolithic column. The assay was linear in the concentration range of 100 - 5000 ng mL-1. The mean recovery was 83%. Intra- and inter-day precision were < 9.5% and < 8.9%, respectively and accuracy was in the range ± 8.33%. The method was successfully applied to a bioequivalence study.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License

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