A sensitive and robust lc-ms/ms method with monolithic column and electrospray ionization for the quantitation of efavirenz in human plasma: application to a bioequivalence study

Química Nova

Souza Filho, Jose Homero de¹  Bedor, Danilo Cesar Galindo¹  Universidade Federal de Pernambuco, Recife, Brasil¹  Santana, Davi Pereira de¹  Ramos, Virna Ligiane Souza¹  Sousa, Carlos Eduardo Miranda de¹  Gonçalves, Talita Mota¹ 

关键词: efavirenz;    bioequivalence;    LC-MS/MS.;   

DOI  :  10.1590/S0100-40422011000600007

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

An LC-MS/MS method has been developed for the determination of efavirenz (EFZ) in human plasma using hydrochlorothiazide as internal standard (I.S.). An ESI negative mode with multiple reaction-monitoring was used monitoring the transitions m/z 313.88→69.24 (EFZ) and 296.02→204.76 (I.S.). Samples were extracted using liquid-liquid extraction. The total run time was 2.0 min. The separation was achieved with HPLC-RP using a monolithic column. The assay was linear in the concentration range of 100 - 5000 ng mL-1. The mean recovery was 83%. Intra- and inter-day precision were < 9.5% and < 8.9%, respectively and accuracy was in the range ± 8.33%. The method was successfully applied to a bioequivalence study.

【 授权许可】

Unknown