Development and validation of a high performance liquid chromatographic method for determination of cyclosporine-A from biodegradable intraocular implants

Química Nova

Juliana Barbosa Saliba²  Armando Da Silva Cunha Junior²  Elionai Cassiana De Lima Gomes²  Herman Sander Mansur¹  Gisele Rodrigues Da Silva¹ 

[1] ,Universidade Federal de Minas Gerais Faculdade de Farmácia Belo Horizonte MG ,Brasil

关键词: cyclosporine-A;    biodegradable intraocular implant;    validation;   

DOI  :  10.1590/S0100-40422011000100026

来源: SciELO

【 摘 要 】

An HPLC method was developed and validated aiming to quantify the cyclosporine-A incorporated into intraocular implants, released from them; and in direct contact with the degradation products of PLGA. The separation was carried out in isocratic mode using acetonitrile/water (70:30) as mobile phase, a C18 column at 80 ºC and UV detection at 210 nm. The method provided selectivity based on resolution among peaks. It was linear over the range of 2.5-40.0 µg/mL. The quantitation and detection limits were 0.8 and 1.2 µg/mL, respectively. The recovery was 101.8% and intra-day and inter-day precision was close to 2%.

【 授权许可】

CC BY-NC   
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