Uso de equações lineares na determinação dos parâmetros de Michaelis-Menten

Química Nova

Nakédia M. F. Carvalho²  Bianca M. Pires²  Octavio A. C. Antunes²  Roberto B. Faria²  Renata E. H. M. B. Osório¹  Clovis Piovezan¹  Ademir Neves¹ 

[1] ,Universidade Federal do Rio de Janeiro Instituto de Química Rio de Janeiro RJ ,Brasil

关键词: Michaelis-Menten;    Lineweaver-Burk;    nonlinear least-square fit;   

DOI  :  10.1590/S0100-40422010000700033

来源: SciELO

【 摘 要 】

The Michaelis-Menten equation is used in many biochemical and bioinorganic kinetic studies involving homogeneous catalysis. Otherwise, it is known that determination of Michaelis-Menten parameters K M, Vmax, and k cat by the well-known Lineweaver-Burk double reciprocal linear equation does not produce the best values for these parameters. In this paper we present a discussion on different linear equations which can be used to calculate these parameters and we compare their results with the values obtained by the more reliable nonlinear least-square fit.

【 授权许可】

CC BY-NC   
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