Prediction of Ethylene Production Rate of Barangan Banana Using Kinetic Enzymatic Michaelis-Menten

1st International Conference on Mechanical Electronic and Biosystem Engineering

Rahman, A.F.^1 ; Darmawaty, E.^2 ; Ahmad, U.^2

Major Post-Harvest Technology, Post-Graduated School, Bogor Agriculture University, Dramaga-Bogor, Indonesia^1

Mechanical Engineering and Biosystem Department, Bogor Agriculture University, Dramaga-Bogor, Indonesia^2

关键词: Enzymatic kinetic;    Ethylene production;    Michaelis-Menten;    Model parameters;    Rate of reaction;    Respiration rate;    Substrate concentrations;    Temperature dependence;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/557/1/012071/pdf
DOI  :  10.1088/1757-899X/557/1/012071

来源: IOP

【 摘 要 】

MM is a model that describes the rate of reaction of product ethylene towards the substrate concentration and MMC is an enzymatic kinetics model to see the effect of the increase of O2 on the rate of reaction. The objective of the study was to estimate ethylene production rate in bananas by applying the Michaelis-Menten enzymatic kinetics equation. Barangan Banana with harvest age 11 and 10 weeks were measured respiration rate with continuous gas analyzer in temperature 10, 20, and 28 °C with 3 replications along measurement of ethylene production rate using ethylene meter obtained from a closed system method and represented by Michael-Menten equation. The temperature dependence was represented base on Arrhenius law. The model parameters (KmC2H4 dan rC2H4max) are calculated using Lineweaver-Burk linier graph method. MM and MMC applications can be used to estimate ethylene production rate of Barangan banana. MM equation resulted that 11-week harvest age obtaining R2 was higher than 10 weeks 0.95 while the 10-week harvested R2 0.94. MMC equation, the 11-week harvest age R2 0.95 was higher compared to 10 weeks R2 0.94. It could be predicted that MM and MMC equations can predicted the rate of ethylene production of Barangan banana.