Imobilização da lacase em micropartículas de quitosana obtidas por spray drying e usadas na construção de biossensores

Química Nova

Inês Rosane Welter Zwirtes De Oliveira²  Orlando Fatibello-filho²  Suellen Cadorin Fernandes¹  Iolanda Cruz Vieira¹ 

[1] ,Universidade Federal de São Carlos Departamento de Química São Carlos SP ,Brasil

关键词: spray dryer;    microparticles;    biosensor;   

DOI  :  10.1590/S0100-40422009000500021

来源: SciELO

【 摘 要 】

Biosensors based on laccase immobilized on microparticles of chitosan crosslinked with tripolyphosphate (biosensor I) and glyoxal (biosensor II) obtained by spray drying for the determinations of rutin in pharmaceutical formulations were developed. Under optimized operational conditions (pH 4.0, frequency of 30 Hz, pulse amplitude of 40 mV and scan increment of 2.0 mV) two analytical curves were obtained for both biosensors showing a detection limit of 6.2x10-8 mol L-1 for biosensor (I) and 2.0x10-8 mol L-1 for biosensor (II). The recovery of rutin from pharmaceutical sample ranged from 90.7 to 105.0% and the lifetime of these biosensors were 4 months (at least 400 determinations).

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License