Immobilization of laccase in microparticles of chitosan obtained by spray drying and used in the biosensors construction

Quimica nova

Fernandes, Suellen Cadorin¹  Vieira, Iolanda Cruz¹  Oliveira, Inês Rosane Welter Zwirtes de²  Fatibello-Filho, Orlando² 

[1] Universidade Federal de Santa Catarina, Florianópolis, Brasil;Universidade Federal de São Carlos, São Carlos, Brasil

关键词: spray dryer;    microparticles;    biosensor.;   

DOI  :  10.1590/S0100-40422009000500021

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Biosensors based on laccase immobilized on microparticles of chitosan crosslinked with tripolyphosphate (biosensor I) and glyoxal (biosensor II) obtained by spray drying for the determinations of rutin in pharmaceutical formulations were developed. Under optimized operational conditions (pH 4.0, frequency of 30 Hz, pulse amplitude of 40 mV and scan increment of 2.0 mV) two analytical curves were obtained for both biosensors showing a detection limit of 6.2x10-8 mol L-1 for biosensor (I) and 2.0x10-8 mol L-1 for biosensor (II). The recovery of rutin from pharmaceutical sample ranged from 90.7 to 105.0% and the lifetime of these biosensors were 4 months (at least 400 determinations).

【 授权许可】

CC BY