Desenvolvimento de metodologia analítica para determinação de colesterol em ração para ruminantes através de planejamento experimental fatorial

Química Nova

Cecília Muller Bandeira²  José Maria Ferreira²  Neura Bragagnolo¹  Lilian Regina Barros Mariutti¹ 

[1] ,Universidade Federal de Minas Gerais Escola de Veterinária Departamento de Tecnologia e Inspeção de Produtos de Origem AnimalBelo Horizonte MG ,Brasil

关键词: GC;    extraction;    validation;   

DOI  :  10.1590/S0100-40422008000600027

来源: SciELO

【 摘 要 】

A chromatographic method was developed for cholesterol determination in feed for ruminants using response surface methodology. Among the five approaches of sample preparation methods tested, the saponification of the sample without heating presented less interference in the gas chromatography. The method presented a relative standard deviation (RSD) of 4.3%, recoveries between 84 and 87% and detection limit of 0.001 mg of cholesterol per g of feed.

【 授权许可】

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