Development of an analytical method for cholesterol determination in feed for ruminants using factorial experimental design

Química Nova

Ferreira, José Maria¹  Mariutti, Lilian Regina Barros¹  Bandeira, Cecília Muller¹  Universidade Federal de Minas Gerais, Belo Horizonte, Brasil¹  Universidade Estadual de Campinas, Campinas, Brasil¹  Bragagnolo, Neura¹ 

关键词: GC;    extraction;    validation.;   

DOI  :  10.1590/S0100-40422008000600027

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

A chromatographic method was developed for cholesterol determination in feed for ruminants using response surface methodology. Among the five approaches of sample preparation methods tested, the saponification of the sample without heating presented less interference in the gas chromatography. The method presented a relative standard deviation (RSD) of 4.3%, recoveries between 84 and 87% and detection limit of 0.001 mg of cholesterol per g of feed.

【 授权许可】

Unknown