Determination of etoricoxib in human plasma using automated on-line solid-phase extraction coupled with LC-APCI/MS/MS

Química Nova

Sérgio Luiz Dalmora¹  Liberato Brum Junior¹  Ricardo Machado Ferretto¹  Paulo Renato De Oliveira¹  Thiago Barth¹  Maximiliano Da Silva Sangoi¹ 

[1] ,Universidade Federal de Santa Maria Centro de Ciências da Saúde Departamento de Farmácia IndustrialSanta Maria RS ,Brazil

关键词: etoricoxib;    LC-APCI/MS/MS;    on-line solid-phase extraction;   

DOI  :  10.1590/S0100-40422008000300021

来源: SciELO

【 摘 要 】

A liquid chromatography-tandem mass spectrometry method with atmospheric pressure chemical ionization (LC-APCI/MS/MS) was validated for the determination of etoricoxib in human plasma using antipyrin as internal standard, followed by on-line solid-phase extraction. The method was performed on a Luna C18 column and the mobile phase consisted of acetonitrile:water (95:5, v/v)/ammonium acetate (pH 4.0; 10 mM), run at a flow rate of 0.6 mL/min. The method was linear in the range of 1-5000 ng/mL (r²>0.99). The lower limit of quantitation was 1 ng/mL. The recoveries were within 93.72-96.18%. Moreover, method validation demonstrated acceptable results for the precision, accuracy and stability studies.

【 授权许可】

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