Determination of etoricoxib in human plasma using automated on-line solid-phase extraction coupled with LC-APCI/MS/MS

Quimica nova

Sangoi, Maximiliano da Silva¹  Dalmora, Sérgio Luiz¹  Brum Junior, Liberato¹  Ferretto, Ricardo Machado¹  Oliveira, Paulo Renato de¹  Barth, Thiago¹ 

[1] Universidade Federal de Santa Maria, Santa Maria, Brazil

关键词: etoricoxib;    LC-APCI/MS/MS;    on-linesolid-phase extraction.;   

DOI  :  10.1590/S0100-40422008000300021

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

A liquid chromatography-tandem mass spectrometrymethod with atmospheric pressure chemical ionization (LC-APCI/MS/MS) was validatedfor the determination of etoricoxib in human plasma using antipyrin as internalstandard, followed by on-line solid-phase extraction. The method was performedon a Luna C18 column and the mobile phase consisted of acetonitrile:water(95:5, v/v)/ammonium acetate (pH 4.0; 10 mM), run at a flow rate of 0.6 mL/min.The method was linear in the range of 1-5000 ng/mL (r2>0.99).The lower limit of quantitation was 1 ng/mL. The recoveries were within 93.72-96.18%.Moreover, method validation demonstrated acceptable results for the precision,accuracy and stability studies.

【 授权许可】

CC BY