Otimização de metodologia para derivação de desoxinivalenol através de planejamento experimental

Química Nova

Jaqueline Garda¹  Eliana Badiale-furlong¹ 

[1] ,Fundação Universidade Federal do Rio Grande Departamento de Química Rio Grande RS ,Brasil

关键词: deoxynivalenol;    derivatization;    methodology;   

DOI  :  10.1590/S0100-40422008000200015

来源: SciELO

【 摘 要 】

The objective of this work was to optimize the derivatization reaction for determining deoxynivalenol (DON) by gas chromatography employing an experimental planning procedure. The factors were: temperature, reaction time, catalyst and trifluoroacetic anhydride concentration. The relative peak areas were used to evaluated the effects. The best conditions for DON derivatization were 200 µL TFAA and 18 mg sodium bicarbonate for 6 min at 74 ºC for 7 to 21 µg of DON. Under these conditions, the detection limit was 1.4 µg of DON.

【 授权许可】

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