Optimization of the methodology for deoxynivalenol derivatization by experimental planning

Química Nova

Badiale-Furlong, Eliana¹  Fundação Universidade Federal do Rio Grande, Rio Grande, Brasil¹  Garda, Jaqueline¹ 

关键词: deoxynivalenol;    derivatization;    methodology.;   

DOI  :  10.1590/S0100-40422008000200015

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

The objective of this work was to optimize the derivatization reaction for determining deoxynivalenol (DON) by gas chromatography employing an experimental planning procedure. The factors were: temperature, reaction time, catalyst and trifluoroacetic anhydride concentration. The relative peak areas were used to evaluated the effects. The best conditions for DON derivatization were 200 µL TFAA and 18 mg sodium bicarbonate for 6 min at 74 ºC for 7 to 21 µg of DON. Under these conditions, the detection limit was 1.4 µg of DON.

【 授权许可】

Unknown