Extração de esterase de fígado suíno (PLE)

Química Nova

Henrique Celso Trevisan¹  João Batista De Medeiros¹  Helen Cristina Fávero Lisboa¹ 

[1] ,Universidade Estadual Paulista Julio de Mesquita Filho Instituto de Química Departamento de Bioquímica e Tecnologia QuímicaAraraquara SP ,Brasil

关键词: porcine liver esterase;    PLE;    biocatalysis;   

DOI  :  10.1590/S0100-40422006000400040

来源: SciELO

【 摘 要 】

A simple, fast and low-cost methodology was optimized, seeking preparation of a crude pig liver esterase (PLE) concentrate. Basically, the method consisted of the following steps: liver homogenization, acetone washing, enzyme extraction and purification/concentration. Starting from 1 kg of fresh liver more than 200 kU of PLE suspension were obtained after 8 hours, at an estimated cost of US$0.21/kU. The PLE concentrate thus obtained was stable, showing 96-100% of the initial activity after 7 months in refrigerator at 4°C.

【 授权许可】

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