Pig liver esterase (PLE) extraction

Quimica nova

Trevisan, Henrique Celso¹  Medeiros, João Batista de¹  Lisboa, Helen Cristina Fávero¹ 

[1] Universidade Estadual Paulista Julio de Mesquita Filho, Araraquara, Brasil

关键词: porcine liver esterase;    PLE;    biocatalysis;   

DOI  :  10.1590/S0100-40422006000400040

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

A simple, fastand low-cost methodology was optimized, seeking preparation of a crude pig liveresterase (PLE) concentrate. Basically, the method consisted of the followingsteps: liver homogenization, acetone washing, enzyme extraction and purification/concentration.Starting from 1 kg of fresh liver more than 200 kU of PLE suspension were obtainedafter 8 hours, at an estimated cost of US$0.21/kU. The PLE concentrate thusobtained was stable, showing 96-100% of the initial activity after 7 monthsin refrigerator at 4°C.

【 授权许可】

CC BY