Estudo espectroscópico do equilíbrio entre espécies hexacoordenada e pentacoordenada dos monômero d nativo e reconstituído da hemoglobina extracelular gigante de Glossoscolex paulistus em meio alcalino

Química Nova

Julio C. Ribelatto¹  Alessandra L. Poli¹  Leonardo M. Moreira¹  Hidetake Imasato¹ 

[1] ,Universidade de São Paulo Instituto de Química de São Carlos São Carlos SP ,Brasil

关键词: extracellular hemoglobin;    hexacoordinated;    pentacoordinated;   

DOI  :  10.1590/S0100-40422006000400008

来源: SciELO

【 摘 要 】

UV-Vis and fluorescence spectroscopic studies of native and reconstituted d monomers from Glossoscolex paulistus were performed in alkaline pH. In addition to the presence of aquomet and hemichrome hexacoordinated species, a pentacoordinated species occurs. This latter species, which presents an imidazolate group of axial histidine as fifth ligand, is identified by the blue-shifted low-intensity Soret band and LMCT band. The pentacoordinated species must occur due to partial polypeptide unfolding.

【 授权许可】

CC BY-NC   
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