Spectroscopic study of the equilibrium between hexacoordinated and pentacoordinated species of the native and reconstituted d monomers of the Glossoscolex paulistus giant extracellular hemoglobin in alkaline medium

Química Nova

Universidade de São Paulo, São Carlos, Brasil¹  Poli, Alessandra L.¹  Ribelatto, Julio C.¹  Imasato, Hidetake¹  Moreira, Leonardo M.¹ 

关键词: extracellular hemoglobin;    hexacoordinated;    pentacoordinated.;   

DOI  :  10.1590/S0100-40422006000400008

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

UV-Vis and fluorescence spectroscopic studies of native and reconstituted d monomers from Glossoscolex paulistus were performed in alkaline pH. In addition to the presence of aquomet and hemichrome hexacoordinated species, a pentacoordinated species occurs. This latter species, which presents an imidazolate group of axial histidine as fifth ligand, is identified by the blue-shifted low-intensity Soret band and LMCT band. The pentacoordinated species must occur due to partial polypeptide unfolding.

【 授权许可】

Unknown