Desenvolvimento de colunas cromatográficas de meios de acesso restrito proteína-imobilizada e suas avaliações para análise de fármacos com injeção direta de plasma humano

Química Nova

Virgínia Veronica De Lima¹  Neila Maria Cassiano¹  Quezia Bezerra Cass¹ 

[1] ,Universidade Federal de São Carlos Departamento de Química São Carlos SP

关键词: RAM column;    direct injection;    plasma samples;   

DOI  :  10.1590/S0100-40422006000100015

来源: SciELO

【 摘 要 】

A series of bovine serum albumin-immobilized supports have been prepared and used as restricted access media (RAM) columns. Restricted-access supports combine size-exclusion of proteins and other high-molar-mass matrix components with the simultaneous enrichment of low-molar mass analytes. These characteristics were chromatographically evaluated for the columns. The RAM-BSA (Bovine Serum Albumin) columns showed excellent performance for exclusion of human plasma protein with good retention capacity for a series of acidic, basic, and neutral drugs.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License