Development of restricted-access media chromatographic columns protein-immobilized and their evaluation for the analysis of drugs by direct injection of human plasma

Química Nova

Cassiano, Neila Maria¹  Cass, Quezia Bezerra¹  Universidade Federal de São Carlos, São Carlos¹  Lima, Virgínia Veronica de¹ 

关键词: RAM column;    direct injection;    plasma samples.;   

DOI  :  10.1590/S0100-40422006000100015

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

A series of bovine serum albumin-immobilized supports have been prepared and used as restricted access media (RAM) columns. Restricted-access supports combine size-exclusion of proteins and other high-molar-mass matrix components with the simultaneous enrichment of low-molar mass analytes. These characteristics were chromatographically evaluated for the columns. The RAM-BSA (Bovine Serum Albumin) columns showed excellent performance for exclusion of human plasma protein with good retention capacity for a series of acidic, basic, and neutral drugs.

【 授权许可】

Unknown