Caracterização de quitosana por Cromatografia de Permeação em Gel - Influência do método de preparação e do solvente

Química Nova

Kathya Mª Nilza De Carvalho Canella¹  Rosangela Balaban Garcia¹ 

[1] ,Universidade Federal do Rio Grande do Norte Departamento de Química Natal RN

关键词: chitin;    chitosan;    gel permeation chromatography;   

DOI  :  10.1590/S0100-40422001000100004

来源: SciELO

【 摘 要 】

In this work, samples of chitosan obtained in different conditions were characterized by molecular weight distribution, using Gel Permeation Chromatography (GPC), in two different solvents. It was observed that the increase in the number of deacetylation steps promotes a increase in the degree of deacetylation followed by a decrease in the average of molecular weight and polydispersion. The GPC curves obtained for chitosan samples in the two solvents used (CH3COOH 0.30 mol/dm³ - CH3CONa 0.20 mol/dm³ and CH3COOH 0.10 mol/dm³ - NaCl 0.20 mol/dm³) showed small difference in elution volume, but significant changes in the average molecular weight (Mn and Mw) and polydispersion that, in agree with the values of Huggins constant, present evidences of chitosan aggregates formation in the second solvent.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License