Determinação espectrofotométrica de aspartame em adoçantes por injeção em fluxo usando um reator em fase sólida contendo fosfato de zinco imobilizado

Química Nova

Airton Vicente Pereira²  Luiz Humberto Marcolino-junior¹  Orlando Fatibello-filho¹ 

[1] ,Universidade Estadual de Ponta Grossa Setor de Ciências Biológicas e da Saúde Departamento de Ciências FarmacêuticasPonta Grossa PR

关键词: aspartame;    flow injection spectrophotometry;    solid-phase reactor;    sweeteners;   

DOI  :  10.1590/S0100-40422000000200005

来源: SciELO

【 摘 要 】

A flow injection spectrophotometric method was developed for determining aspartame in sweeteners. Sample was dissolved in water and 250 µL of the solution was injected into a carrier stream of 5.0 x 10-5 mol L-1 sodium borate solution. The sample flowed through a column (14 cm x 2.0 mm) packed with Zn3(PO4)2 immobilized in a polymeric matrix of polyester resin and Zn(II) ions were released from the solid-phase reactor by formation of the Zn(II)-aspartame complex. The mixture merged with a stream of borate buffer solution (pH 9.0) containing 0.030 % (m/v) alizarin red S and the Zn(II)-alizarin red complex formed was measured spectrophotometrically at 540 nm. The calibration graph for aspartame was linear in the concentration range from 10 to 80 µg mL-1 with a detection limit of 4 µg mL-1 of aspartame. The RSD was 0.3 % for a solution containing 40 µg mL-1 aspartame (n = 10) and seventy results were obtained per hour. The proposed method was applied for determining aspartame in commercial sweeteners.

【 授权许可】

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