Optimization of heterologous microsatellites in Piracanjuba

Pesquisa Agropecuária Brasileira

Fausto Moreira Da Silva Carmo¹  Érico Macedo Polo¹  Marcos Antônio Da Silva¹  Gabriel De Menezes Yazbeck¹ 

关键词: Brycon orbignyanus;    aquaculture;    hatchery;    molecular markers;    Brycon orbignyanus;    aquacultura;    procriação;    marcadores moleculares;   

DOI  :  10.1590/S0100-204X2015001200015

来源: SciELO

【 摘 要 】

Abstract: The objective of this work was to evaluate 41 microsatellite markers for heterologous amplifications in piracanjuba (Brycon orbignyanus). Some markers were tested for the first time. Loci were optimized for PCR conditions and applied to a sample of 49 individuals. Thirty-one loci resulted in PCR product formation, whereas ten loci yielded intelligible polymorphic patterns in the evaluated sample and can be used for amplifications in this species. From the evaluated markers, four loci (BoM1, BoM13, Bh6, and Bh16) are valid to be applied in the study of piracanjuba.

【 授权许可】

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