Reaction of sweet orange cultivars expressing the attacin A gene to 'Candidatus Liberibacter asiaticus' infection

Pesquisa Agropecuária Brasileira

Rafaella Teles Arantes Felipe²  Francisco De Assis Alves Mourão Filho²  Silvio Aparecido Lopes¹  Beatriz Madalena Januzzi Mendes¹  Maurel Behling¹  Ernani Vicente Pereira Junior² 

[1] ,Universidade de São Paulo Escola Superior de Agricultura Luiz de Queiroz Piracicaba SP ,Brazil

关键词: Citrus sinensis;    antibacterial peptide;    disease resistance;    genetic transformation;    huanglongbing;    Citrus sinensis;    peptídeo antibacteriano;    resistência a doenças;    transformação genética;    huanglongbing;   

DOI  :  10.1590/S0100-204X2013001100004

来源: SciELO

【 摘 要 】

The objective of this work was to evaluate the reaction of four sweet orange cultivars expressing the attacin A gene to 'Candidatus Liberibacter asiaticus' (Las) infection, a bacterium associated to huanglongbing (HLB) disease. Transgenic sweet orange plants of Hamlin, Natal, Pêra, and Valência cultivars, as well as nontransgenic controls received inocula by grafting budwood sections of HLB-infected branches. Disease progression was evaluated through observations of leaf symptoms and by polymerase chain reaction (PCR) analysis, eight months after inoculation. A completely randomized design was used, with four experiments (one for each cultivar) performed simultaneously. Bacteria title was estimated by quantitative PCR (qPCR). HLB symptoms and Las titers were present in nontransgenic and transgenic plants expressing the attacin A gene of the four sweet orange cultivars, eight months after bacteria inoculation. Five transgenic lines (transformation events) of 'Pêra' sweet orange expressing the attacin A gene have significantly lower Las titers in comparison with nontransgenic plants of this cultivar.

【 授权许可】

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