Selection of shrimp breeders free of white spot syndrome and infectious hypodermal and hematopoietic necrosis

Pesquisa Agropecuária Brasileira

Carlos Cesar De Mello Junior²  Gael Yvan Leclercq Delsol²  Emmerik Motte²  Virna Alexia Cedeño Escobar²  Pedro Filipe Rey²  Mauricio Laterça Martins¹  Luis Alejandro Vinatea Arana¹  Giovanni Lemos De Mello¹  Alvaro Pestana De Farias¹  Xavier Antonio Serrano Arguello²  John Erick Montaño Maridueña² 

[1] ,Concepto Azul, CdlaGuayaquil,Ecuador

关键词: Litopenaeus vannamei;    breeding;    epidemiology;    nested-PCR;    Litopenaeus vannamei;    reprodução;    epidemiologia;    nested-PCR;   

DOI  :  10.1590/S0100-204X2011000500011

来源: SciELO

【 摘 要 】

The objective of this work was to select surviving breeders of Litopenaeus vannamei from white spot syndrome virus (WSSV) outbreak, adapted to local climatic conditions and negatively diagnosed for WSSV and infectious hypodermal and hematopoietic necrosis virus (IHHNV), and to evaluate if this strategy is a viable alternative for production in Santa Catarina, Brazil. A total of 800 males and 800 females were phenotypically selected in a farm pond. Nested-PCR analyses of 487 sexually mature females and 231 sexually mature males showed that 63% of the females and 55% of the males were infected with IHHNV. Animals free of IHHNV were tested for WSSV, and those considered double negative were used for breeding. The post-larvae produced were stocked in nine nursery tanks for analysis. From the 45 samples, with 50 post-larvae each, only two were positive for IHHNV and none for WSSV. Batches of larvae diagnosed free of virus by nested-PCR were sent to six farms. A comparative analysis was carried out in growth ponds, between local post-larvae and post-larvae from Northeast Brazil. Crabs (Chasmagnathus granulata), blue crabs (Callinectes sapidus), and sea hares (Aplysia brasiliana), which are possible vectors of these viruses, were also evaluated. The mean survival was 55% for local post-larvae against 23.4% for post-larvae from the Northeast. Sea hares showed prevalence of 50% and crabs of 67% of WSSV.

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