Stability of Citrus tristeza virus protective isolates in field conditions

Pesquisa Agropecuária Brasileira

Alessandra Tenório Costa¹  William Mário De Carvalho Nunes¹  Carlos Alexandre Zanutto¹  Gerd Walter Müller¹ 

[1] ,Universidade Estadual de Maringá Núcleo de Pesquisa em Biotecnologia Aplicada Maringá PR ,Brazil

关键词: Citrus sinensis;    cross-protection;    preimmunization;    single strand conformational polymorphism;    stem pitting symptoms;    Citrus sinensis;    proteção cruzada;    pré-imunização;    polimorfismo conformacional da fita simples;    sintomas de caneluras;   

DOI  :  10.1590/S0100-204X2010000700009

来源: SciELO

【 摘 要 】

The objective of this work was to monitor the maintenance of Citrus tristeza virus (CTV) protective isolates stability in selected clones of 'Pêra' sweet orange (Citrus sinensis), preimmunized or naturally infected by the virus, after successive clonal propagations. The work was carried out in field conditions in the north of Paraná State, Brazil. Coat protein gene (CPG) analysis of 33 isolates collected from 16 clones of 'Pêra' sweet orange was performed using single strand conformational polymorphism (SSCP). Initially, the isolates were characterized by symptoms of stem pitting observed in clones. Then viral genome was extracted and used as template for the amplification of CPG by reverse transcription polimerase chain reaction (RTPCR). RTPCR products electrophoretic profiles were analyzed using the Jaccard coefficient and the UPGMA method. The majority of the clones had weak to moderate stem pitting symptoms and its CTV isolates showed alterations in the SSCP profiles. However, the stability of the protective complex has been maintained, except for isolates from two analised clones. Low genetic variability was observed within the isolates during the studied years.

【 授权许可】

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