Fusion of protoplasts with irradiated microprotoplasts as a tool for radiation hybrid panel in citrus

Pesquisa Agropecuária Brasileira

Claudine Maria De Bona²  David Stelly¹  J. Creighton Miller Jr.¹  Eliezer Silva Louzada¹ 

[1] ,Instituto Agronômico do Paraná Centro Administrativo do Governo do Estado Curitiba PR ,Brazil

关键词: Citrus;    Swinglea glutinosa;    asymmetric somatic hybridization;    gamma rays;    hybrid cell lines;    radiation hybrid mapping;    Citrus;    Swinglea glutinosa;    hibridação somática assimétrica;    raios gama;    linhagens de células híbridas;    mapeamento de híbridos por radiação;   

DOI  :  10.1590/S0100-204X2009001200008

来源: SciELO

【 摘 要 】

The objective of this work was to combine asymmetric somatic hybridization (donor-recipient fusion or gamma fusion) to microprotoplast-mediated chromosome transfer, as a tool to be used for chromosome mapping in Citrus. Swinglea glutinosa microprotoplasts were irradiated either with 50, 70, 100 or 200 gamma rays and fused to cv. Ruby Red grapefruit or Murcott tangor protoplasts. Cell colonies were successfully formed and AFLP analyses confirmed presence of S. glutinosa in both 'Murcott' tangor and 'Ruby Red' grapefruit genomes.

【 授权许可】

CC BY   
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