Kinetic parameters of soil β-glucosidase response to environmental temperature and moisture regimes

Revista Brasileira de Ciência do Solo

Yulan Zhang¹  Lijun Chen¹  Zhijie Wu¹  Caixia Sun² 

[1] ,Northeastern University of China

关键词: enzymatic kinetic;    soil moisture;    cellulose biodegradation;    cinética enzimática;    água no solo;    biodegradação de celulose;   

DOI  :  10.1590/S0100-06832011000400022

来源: SciELO

【 摘 要 】

Soil β-glucosidase participates in the final step of cellulose biodegradation. It is significant in the soil C cycle and is used as an indicator of the biological fertility of soil. However, the response of its kinetic parameters to environmental temperature and moisture regimes is not well understood. This study tested the β-glucosidase response in the main agricultural soils (black soil, albic soil, brown soil, and cinnamon soil) of Northeast China. Incubation tests were conducted to measure the kinetic parameters Km, Vmax or Vmax/Km of soil β-glucosidase at environmental temperatures of 10, 20 and 30 ºC and at 10, 20 and 30 % soil moisture content. The insensitive response of the kinetic parameters to temperature changes indicates that soil β-glucosidase was present primarily in immobilized form. The significant response of the kinetic parameters of soil β-glucosidase to soil moisture rather than to environmental temperatures suggests that the catalytic ability of soil β-glucosidase was sensitive to changing soil moisture regimes.

【 授权许可】

CC BY   
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