Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil

Memórias do Instituto Oswaldo Cruz

Jefferson José Da Silva Santos¹  Tereza Magalhães¹  José Valter Joaquim Silva Junior¹  Andréa Nazaré Monteiro Rangel Da Silva¹  Marli Tenório Cordeiro¹  Laura Helena Vega Gonzales Gil¹ 

关键词: infectious clone;    yeast homologous recombination;    flavivirus;   

DOI  :  10.1590/0074-02760150053

来源: SciELO

【 摘 要 】

Full-length dengue virus (DENV) cDNA clones are an invaluable tool for many studies, including those on the development of attenuated or chimeric vaccines and on host-virus interactions. Furthermore, the importance of low passage DENV infectious clones should be highlighted, as these may harbour critical and unique strain-specific viral components from field-circulating isolates. The successful construction of a functional Brazilian low passage DENV serotype 2 full-length clone through homologous recombination reported here supports the use of a strategy that has been shown to be highly useful by our group for the development of flavivirus infectious clones and replicons.

【 授权许可】

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