Identification and Inheritance of New Sources of Resistance against Cucumber mosaic virus Isolate P1 in Capsicum and Development of a Tomato yellow leaf curl Kanchanaburi virus Infectious Clone

Cucumber mosaic virus (CMV);Tomato yellow leaf curl Kanchanaburk virus (TYLCKaV);infectious clone;Capsicum;Pepper virus;633

농업생명과학대학 식물생산과학부 ;

University:서울대학교 대학원

关键词: Cucumber mosaic virus (CMV);    Tomato yellow leaf curl Kanchanaburk virus (TYLCKaV);    infectious clone;    Capsicum;    Pepper virus;    633;   

Others  :  http://s-space.snu.ac.kr/bitstream/10371/141819/3/000000150452.pdf

美国|英语

来源: Seoul National University Open Repository

【 摘 要 】

Peppers (Capsicum spp.) are important vegetable and spice crop worldwide. It possesses rich accumulations of secondary metabolites, colors and certain essential vitamins and minerals. Viral diseases are severe threat to both hot and sweet peppers resulting in significant yield loss. In the present study, two pepper viruses causing such excessive yield and quality loss of pepper were studied. In the first chapter, Capsicum germplasm collection was screened to identify resistance sources of CMV-P1, a new variant of Cucumber mosaic virus (CMV) strain. No accumulation of the virus in resistant pepper accessions was verified by enzyme linked immunosorbent assay (ELISA). Among the 4,197 accessions, seven accessions of C. annuum have been screened out as new resistant sources. Pepper accessions exhibiting strong resistance against CMV-P1 were used to develop F1 population and each of them was crossed with a susceptible line C. annuum ;;Jeju’ and a resistance line C. annuum ;;Lam32’, containing cmr2. The developed populations were further utilized to identify the inheritance of genetic resistance. The results suggest that the identified seven resistant accessions showed an identical pattern of genetic inheritance as the resistance gene, cmr2. The resistant resources identified in this study are expected to be applied in pepper breeding to develop CMV-P1 resistant varieties. In the second chapter, the genome of Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV), a bipartite Begomovirus first reported to infect tomato and eggplant in Kanchanaburi (Thailand), was sequenced and confirmed. A TYLCKaV infectious clone was developed using the golden gate cloning method and was agroinfiltrated in tobacco, tomato and pepper plants. The virus inoculated tobacco and tomato plants displayed viral symptoms. However, no obvious TYLCKaV symptoms were observed in pepper. Accumulation of the virus was also confirmed using polymerase chain reaction (PCR). The developed TYLCKaV infectious clone will be a useful tool for studies on TYLCKaV resistance.