Experimental benznidazole treatment of Trypanosoma cruzi II strains isolated from children of the Jequitinhonha Valley, Minas Gerais, Brazil, with Chagas disease

Memórias do Instituto Oswaldo Cruz

Jaquelline Carla Valamiel De Oliveira-silva¹  Girley Francisco Machado-de-assis¹  Maykon Tavares Oliveira¹  Nívia Carolina Noguieira Paiva¹  Márcio Sobreira Silva Araújo¹  Cláudia Martins Carneiro¹  Olindo Assis Martins-filho¹  Helen Rodrigues Martins¹  Marta De Lana¹ 

关键词: Trypanosoma cruziII;    benznidazole response;    acute and chronic phases;    murine model;    Jequitinhonha Valley;    MG;    Brazil;   

DOI  :  10.1590/0074-02760140260

来源: SciELO

【 摘 要 】

Trypanosoma cruzi strains from distinct geographic areas show differences in drug resistance and association between parasites genetic and treatment response has been observed. Considering that benznidazole (BZ) can reduce the parasite burden and tissues damage, even in not cured animals and individuals, the goal is to assess the drug response to BZ of T. cruzi II strains isolated from children of the Jequitinhonha Valley, state of Minas Gerais, Brazil, before treatment. Mice infected and treated with BZ in both phases of infection were compared with the untreated and evaluated by fresh blood examination, haemoculture, polymerase chain reaction, conventional (ELISA) and non-conventional (FC-ALTA) serologies. In mice treated in the acute phase, a significant decrease in parasitaemia was observed for all strains. Positive parasitological and/or serological tests in animals treated during the acute and chronic (95.1-100%) phases showed that most of the strains were BZ resistant. However, beneficial effect was demonstrated because significant reduction (p < 0.05%) and/or suppression of parasitaemia was observed in mice infected with all strains (acute phase), associated to reduction/elimination of inflammation and fibrosis for two/eight strains. BZ offered some benefit, even in not cured animals, what suggest that BZ use may be recommended at least for recent chronic infection of the studied region.

【 授权许可】

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