Phospholipase gene expression during Paracoccidioides brasiliensis morphological transition and infection

Memórias do Instituto Oswaldo Cruz

Deyze Alencar Soares¹  Marilia Barros Oliveira¹  Adriane Feijo Evangelista¹  Emerson Jose Venancio¹  Rosangela Vieira Andrade¹  Maria Sueli Soares Felipe¹  Silvana Petrofeza¹ 

关键词: host-pathogen interaction;    Paracoccidioides brasiliensis;    pathogenicity;    phospholipases;   

DOI  :  10.1590/0074-0276108062013021

来源: SciELO

【 摘 要 】

Phospholipase is an important virulence factor for pathogenic fungi. In this study, we demonstrate the following: (i) the Paracoccidioides brasiliensis pld gene is preferentially expressed in mycelium cells, (ii) the plb1 gene is mostly up-regulated by infection after 6 h of co-infection of MH-S cells or during BALB/c mice lung infection, (iii) during lung infection, plb1, plc and pld gene expression are significantly increased 6-48 h post-infection compared to 56 days after infection, strongly suggesting that phospholipases play a role in the early events of infection, but not during the chronic stages of pulmonary infection by P. brasiliensis.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License