Trypanosoma rangeli protein tyrosine phosphatase is associated with the parasite's flagellum

Memórias do Instituto Oswaldo Cruz

Elisa Beatriz Prestes²  Ethel Bayer-santos²  Patrícia Hermes Stoco²  Thaís Cristine Marques Sincero²  Glauber Wagner²  Adriana Umaki¹  Stenio Perdigão Fragoso¹  Juliano Bordignon¹  Mário Steindel²  Edmundo Carlos Grisard² 

[1] ,Universidade Federal de Santa Catarina Centro de Ciências Biológicas Departamento de Microbiologia, Imunologia e ParasitologiaFlorianópolis SC ,Brasil

关键词: Trypanosoma rangeli;    protein tyrosine phosphatase;    flagellum;   

DOI  :  10.1590/S0074-02762012000600002

来源: SciELO

【 摘 要 】

Protein tyrosine phosphatases (PTPs) play an essential role in the regulation of cell differentiation in pathogenic trypanosomatids. In this study, we describe a PTP expressed by the non-pathogenic protozoan Trypanosoma rangeli (TrPTP2). The gene for this PTP is orthologous to the T. brucei TbPTP1 and Trypanosoma cruzi (TcPTP2) genes. Cloning and expression of the TrPTP2 and TcPTP2 proteins allowed anti-PTP2 monoclonal antibodies to be generated in BALB/c mice. When expressed by T. rangeli epimastigotes and trypomastigotes, native TrPTP2 is detected as a ~65 kDa protein associated with the parasite's flagellum. Given that the flagellum is an important structure for cell differentiation in trypanosomatids, the presence of a protein responsible for tyrosine dephosphorylation in the T. rangeli flagellum could represent an interesting mechanism of regulation in this structure.

【 授权许可】

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