Immunostimulatory property of a synthetic peptide belonging to the soluble ATP diphosphohydro-lase isoform (SmATPDase 2) and immunolocalisation of this protein in the Schistosoma mansoni egg

Memórias do Instituto Oswaldo Cruz

Rita Gabriela Pedrosa Ribeiro Mendes²  Michélia Antônia Do Nascimento Gusmão¹  Ana Carolina Ribeiro Gomes Maia²  Michelle De Lima Detoni²  Gabriane Nascimento Porcino²  Thais Vieira Soares²  Maria Aparecida Juliano¹  Luiz Juliano¹  Paulo Marcos Zech Coelho¹  Henrique Leonel Lenzi¹  Priscila Faria-pinto²  Eveline Gomes Vasconcelos² 

[1] ,Universidade Federal de Juiz de Fora Instituto de Ciências Biológicas Departamento de BioquímicaJuiz de Fora MG ,Brasil

关键词: Schistosoma mansoni;    NTPDase;    potato apyrase;    peptide;   

DOI  :  10.1590/S0074-02762011000700005

来源: SciELO

【 摘 要 】

A peptide (SmB2LJ; r175-194) that belongs to a conserved domain from Schistosoma mansoni SmATPDase 2 and is shared with potato apyrase, as predicted by in silico analysis as antigenic, was synthesised and its immunostimulatory property was analysed. When inoculated in BALB/c mice, this peptide induced high levels of SmB2LJ-specific IgG1 and IgG2a subtypes, as detected by enzyme linked immunosorbent assay. In addition, dot blots were found to be positive for immune sera against potato apyrase and SmB2LJ. These results suggest that the conserved domain r175-194 from the S. mansoni SmATPDase 2 is antigenic. Western blots were performed and the anti-SmB2LJ antibody recognised in adult worm (soluble worm antigen preparation) or soluble egg antigen antigenic preparations two bands of approximately 63 and 55 kDa, molecular masses similar to those predicted for adult worm SmATPDase 2. This finding strongly suggests the expression of this same isoform in S. mansoni eggs. To assess localisation of SmATPDase 2, confocal fluorescence microscopy was performed using cryostat sections of infected mouse liver and polyclonal antiserum against SmB2LJ. Positive reactions were identified on the external surface from the miracidium in von Lichtenberg's envelope and, in the outer side of the egg-shell, showing that this soluble isoform is secreted from the S. mansoni eggs.

【 授权许可】

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