A transient increase in total head phosphotyrosine levels is observed upon the emergence of Aedes aegypti from the pupal stage

Memórias do Instituto Oswaldo Cruz

Willy Jablonka¹  Raquel Senna¹  Thaisa Nahu¹  Guilherme Ventura¹  Lidiane Menezes¹  Mário Alberto C Silva-neto¹ 

[1] ,Instituto de Bioquímica Médica Laboratório de Sinalização Celular Programa de Biologia Molecular e Biotecnologia

关键词: Aedes aegypti;    tyrosine phosphorylation;    blood-feeding;   

DOI  :  10.1590/S0074-02762011000500005

来源: SciELO

【 摘 要 】

Phosphorylation and dephosphorylation of protein tyrosine residues constitutes a major biochemical regulatory mechanism for the cell. We report a transient increase in the total tyrosine phosphorylation of the Aedes aegypti head during the first days after emergence from the pupal stage. This correlates with an initial reduction in total head protein tyrosine phosphatase (PTP) activity. Similarly, phosphotyrosine (pTyr)-containing bands are seen in extracts prepared from both male and female heads and are spread among a variety of structures including the antennae, proboscis and the maxillary palps combined with the proboscis. Also, mosquitoes treated with sodium orthovanadate, a classical PTP inhibitor, show reduced blood-feeding activity and higher head tyrosine phosphorylation levels. These results suggest that pTyr-mediated signalling pathways may play a role in the initial days following the emergence of the adult mosquito from the pupal stage.

【 授权许可】

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